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COMPARING THREE METHODS OF EXTRACTION OF AURICULARIA AURICULA POLYSACCHARIDES

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Author: Liang Li, Xiaoyu Yang, Lei Pan, Yue Su, Yan Wang

Abstract: Auricularia auricula are rich source of bioactive polysaccharides. Three methods of extraction of polysaccharide from these fungi was studied. These methods included, water-based, ultrasonic-assisted, and enzyme-based extractions. The results showed that the enzyme-based extraction was better than ultrasonic-assisted extraction and water extraction. While abundance of polysaccharide concentration in A. auricula-judae and A. delicata were similar, their yield following enzyme-based extractions were higher than that of A. polytricha. The maximum A. auricula-judae polysaccharide concentration in enzyme-assisted extraction was obtained at a pH of 5.0, a temperature of 50°C and 3% cellulase concentration, and the polysaccharide concentration was 52.28 mg/g. The maximum A. delicata polysaccharide concentration (55.16 mg/g) was extracted at a pH of 5.0, a temperature of 50°C and 4% cellulase concentration.

Keywords: A. auricula, Enzymolysis, Polysaccharides, Ultrasonic, Water extraction

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