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POLYSACCHARIDES FROM POMELO PEELS: EXTRACTION, OPTIMIZATION AND THEIR ANTIOXIDANT ACTIVITY IN VITRO

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Author: Juan Yu, Hai-Yu Ji, Yu-Fang Wang and An-Jun Liu

Abstract: The extraction conditions of water-soluble polysaccharides from pomelo peels were  optimized by response surface methodology. The optimum polysaccharide extraction parameters were determined using a Box-Behnken design as follows: liquid-solid ratio of 34 mL/g, extraction temperature of 87șC, extraction time of 2 h and extraction frequency  of 2. Under these conditions, the corresponding yield of polysaccharides was 8.73%, which was consistent with the predicted value of 8.78%. The in vitro antioxidant assays  revealed that pomelo peels polysaccharides exhibited higher scavenging activity to  1,1-diphenyl-2-picrylhydrazyl radicals, whereas relatively lower scavenging activities  to superoxide anions and hydroxyl radical. Taken together, these results suggested that pomelo peels polysaccharides had the potential to be antioxidants in pharmaceutical  industries and functional foods.

Keywords: Antioxidant activity, Box-Behnken design, Polysaccharides, Pomelo peels

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