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The Protective Effect of Sulfated Pachymaran on 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neuronal Apoptosis in Mice

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Author: Gui-Zhen Gao, Chao Wu, Ke-Feng Zhai, Ling-Ling Shan and Mei-Hong Li

Abstract: Mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine were used to examine the neuroprotective effects of sulfated pachymaran. Male ICR mice were administered 30 mg/kg  of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intraperitoneally once a day for 7 days  in model group. In sulfated pachymaran treatment groups, different doses of sulfated  pachymaran (50, 100, and 150 mg/kg, respectively) were given consecutively after  1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treatment in the following 7 days. The  apoptosis of neurons in substantia nigra was observed by tyrosine hydroxylase  immunostaining and TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end  labeling) staining. Expression of Bcl-2, and Bax proteins in midbrain and striatum were  detected by Western blot. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treatment significantly decreased the tyrosine hydroxylase-immunoreactive neurons (P < 0.01),  increased the TUNEL-positive neurons (P < 0.05).  1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine markedly induced downregulation of Bcl-2 expression (P < 0.05, P < 0.01, respectively) and upregulation of Bax (P < 0.05, P <  0.01, respectively) expression in both midbrain and striatum. Sulfated pachymaran restored 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neuronal death in the nigrostriatal pathway, and significantly elevated Bcl-2 expression and declined Bax  expression, contributing to the balance between Bcl-2 and Bax proteins. These results indicated that sulfated pachymaran had protective effect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced apoptosis in striato-nigral system in vivo. 

Keywords: Apoptosis, MPTP, Parkinson disease, Sulfated pachymaran

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