The Protective Effect of Sulfated Pachymaran on 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neuronal Apoptosis in Mice
Author: Gui-Zhen Gao, Chao Wu, Ke-Feng Zhai, Ling-Ling Shan and Mei-Hong Li
Abstract: Mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine were used to examine the neuroprotective effects of sulfated pachymaran. Male ICR mice were administered 30 mg/kg of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intraperitoneally once a day for 7 days in model group. In sulfated pachymaran treatment groups, different doses of sulfated pachymaran (50, 100, and 150 mg/kg, respectively) were given consecutively after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treatment in the following 7 days. The apoptosis of neurons in substantia nigra was observed by tyrosine hydroxylase immunostaining and TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) staining. Expression of Bcl-2, and Bax proteins in midbrain and striatum were detected by Western blot. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treatment significantly decreased the tyrosine hydroxylase-immunoreactive neurons (P < 0.01), increased the TUNEL-positive neurons (P < 0.05). 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine markedly induced downregulation of Bcl-2 expression (P < 0.05, P < 0.01, respectively) and upregulation of Bax (P < 0.05, P < 0.01, respectively) expression in both midbrain and striatum. Sulfated pachymaran restored 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neuronal death in the nigrostriatal pathway, and significantly elevated Bcl-2 expression and declined Bax expression, contributing to the balance between Bcl-2 and Bax proteins. These results indicated that sulfated pachymaran had protective effect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced apoptosis in striato-nigral system in vivo.
Keywords: Apoptosis, MPTP, Parkinson disease, Sulfated pachymaranOpen/Download