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OPTIMIZATION OF CONDITIONS FOR CHOLESTEROL REMOVAL FROM RANA CHENSINENSIS OVUM OIL

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Author: TIEHUA ZHANG, CHAO ZHAO, CHANGHUI ZHAO, XINHUI ZHOU, YUAN LIANG AND HAIQING YE

Abstract: Chinese forest frog ovum oil is a medicinal food high in cholesterol. In the current study, we evaluated the relative efficiency of ?-cyclodextrin, crosslinked ?-cyclodextrin, and silica gel adsorption on cholesterol removal from Rana chensinensis ovum oil. Beta-cyclodextrin had the highest cholesterol reduction rate (60.84 4.45%) among the three methods. We further evaluated the efficiency of cholesterol removal by beta-cyclodextrin in the presence of ethanol and higher (55C) temperature. This resulted into cholesterol removal rate of as high as 76%. Interestingly, monounsaturated fatty acids increased from 4.96% to 29.12% after cholesterol removal. We conclude that beta-cyclodextrin is an excellent adsorbent for removing cholesterol from ovum oil.

Keywords: Cholesterol, Fatty acids, Rana chensinensis ovum oil, ?-cyclodextrin

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