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OPTIMIZATION OF SUPERCRITICAL FLUID EXTRACTION OF PHENOLIC COMPOUNDS FROM PEACH BLOSSOM (AMYGDALUS PERSICA) BY RESPONSE SURFACE METHODOLOGY

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Author: Zhong-Gao Jiao, Zhengzhou Fruit Research Institute, Zhengzhou, 450009, China. E-mail: jiaozhonggao@caas.cn

Abstract: Supercritical fluid extraction (SFE) was used for the extraction of phenolic compounds from peach blossom (Amygdalus persica). The optimum conditions of SFE were optimized by response surface methodology and confirmed at 64C, 30 MPa, 143 min, and 35 mL ethanol  (100%) as modifier for maximum total phenolic contents (54.10 mg GAE g?1 DW), which was higher than the yield achieved by ultrasonic-assisted extraction (44.04 mg GAE g?1 DW).  The antioxidant activities and tyrosinase inhibitory activities of extraction extracted  by SFE were 79.55% and 14.42%. Furthermore, chlorogenic acid, quercetin-3-O-galactoside, kaempferol-3-O-galactoside, quercitrin-3-O-rhamnoside, and kaempferol 4?-glucoside were identified in this study.

Keywords: Blossom, Peach, Phenolics, Response surface methodology, Supercritical fluid extraction, Tyrosinase inhibitory activity

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