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IDENTIFICATION OF LINEAR IGE EPITOPES FOR A MAJOR ALLERGEN-TYPE I COLLAGEN IN FISH (RAINBOW TROUT)

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Author: YAN-BO WANG, XIAO-HUI LI, JIN-RU ZHOU, YAN ZHANG, AI-JIN MA AND LING-LIN FU

Abstract: Type I collagen was described as a major allergen in fish. The purpose of this study was to screen and identify the linear IgE epitopes of type I collagen  α1 and α2 subunits in  rainbow trout. Five bioinformatics tools were used to predict the potential epitopes and  the resultant epitopes were confirmed by LAD2 cells degranulation assay with sera from  fish allergic patients. As the result, 10 peptides ofα1 and α2 subunits were predicted,  respectively, and these peptides were assembled by solid-phase synthesis. 14 epitopes  were identified by LAD2 cells degranulation assay, among which, peptide 2, 57 were identified as linear epitope of ?1 and peptide 1120 were identified as linear epitope  of ?2. Moreover, for ?1 and ?2 subunits, the similarity of sequences was greater than  79%, suggesting the cross-reactivity of fish collagen. The findings of this study  provided a strong support for further research of reduction of the collagen  allergenicity.

Keywords: ?1 and ?2 subunits; LAD2 cells; Linear epitope; Rainbow trout; Type I collagen

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